PCR detection of periodontopathogenic bacteria

It is known that the physiological flora of the oral cavity consists of hundreds of different types of bacteria. In recent years, in the treatment of aggressive and progressive periodontitis, the use of molecular diagnostics is becoming increasingly important because it is attached to the identification of the most common gram-negative anaerobic periodontopathogenic bacteria.

Standard microbiological tests in dental practice are rarely used due to numerous shortcomings (specific tests of poor quality, sensitivity of samples to transport to the laboratory), today molecular tests are recommended for the diagnosis of periodontitis. The tests are based on the evidence of DNA (deoxyribonucleic

PCR involves the diagnosis of the five most common periodontal bacteria:

Aggregatibacter actinomycetemcomitans

Porphyromonas gingivalis

Prevotella intermedia

Tannerella forsythia

Treponema denticola